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simian virus 40 sv40 large t antigen  (ATCC)


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    Structured Review

    ATCC simian virus 40 sv40 large t antigen
    Simian Virus 40 Sv40 Large T Antigen, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 2531 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/simian+virus+40+sv40+large+t+antigen/Antigen/pm41604784-91-21-32
    Average 97 stars, based on 2531 article reviews
    simian virus 40 sv40 large t antigen - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: In vitro evaluation of anti-HIV radioimmunoconjugates labeled with astatine-211, thorium-227 and actinium-225.
    Article Snippet: We conducted an in vitro investigation of the selective cytotoxicity of alpha-emitting radioimmunoconjugates (α-RICs) directed against cells expressing HIV envelope (Env) proteins.. It is well known that monoclonal antibody (mAb)-targeted α-emitting radionuclides can effectively kill antigen-expressing cells; however, the expected lowlevel expression of HIV antigens on latently infected cells poses an obstacle to all anti-HIV immune-based treatments, including α-RICs.. This investigation tested the cytotoxicity of the HIV envelope antigen-binding mAbs, PGT126 (binding gp120) and 7B2 (binding gp41), conjugated with labeling chelators that bind the α-emitters astatine-211 (211At), actinium-225 (225Ac) or thorium-227 (227Th).

    Modification:

    Article Title: In vitro evaluation of anti-HIV radioimmunoconjugates labeled with astatine-211, thorium-227 and actinium-225.
    Article Snippet: We conducted an in vitro investigation of the selective cytotoxicity of alpha-emitting radioimmunoconjugates (α-RICs) directed against cells expressing HIV envelope (Env) proteins.. It is well known that monoclonal antibody (mAb)-targeted α-emitting radionuclides can effectively kill antigen-expressing cells; however, the expected lowlevel expression of HIV antigens on latently infected cells poses an obstacle to all anti-HIV immune-based treatments, including α-RICs.. This investigation tested the cytotoxicity of the HIV envelope antigen-binding mAbs, PGT126 (binding gp120) and 7B2 (binding gp41), conjugated with labeling chelators that bind the α-emitters astatine-211 (211At), actinium-225 (225Ac) or thorium-227 (227Th).

    Article Title: Epstein-Barr Virus Thymidine Kinase Is a Centrosomal Resident Precisely Localized to the Periphery of Centrioles
    Article Snippet: .. 293 human embryonic kidney cells immortalized by sheared adenovirus (ATCC), 293T human embryonic kidney cells immortalized by sheared adenovirus and stably transfected with simian virus 40 (SV40) large T antigen (ATCC), and 143B TK , a human rhabdomyosarcoma cell line that lacks human TK-1 expression (ATCC), were all grown in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin. .. Chinese hamster ovary K1 (CHO-K1) cells (ATCC) were grown in F-12 medium and similarly supplemented.

    Article Title: Inorganic nanoparticles for transfection of mammalian cells and removal of viruses from aqueous solutions.
    Article Snippet: Owing to their small size, synthetic nanoparticles show unprecedented biophysical and biochemical properties which may foster novel advances in life-science research.. Using flame-spray synthesis technology we have produced non-coated aluminum-, calcium-, cerium-, and zirconium-derived inorganic metal oxide nanoparticles which not only exhibit high affinity for nucleic acids, but can sequester such compounds from aqueous solution.. This non-covalent DNA-binding capacity was successfully used to transiently transfect a variety of mammalian cells including human, reaching transfection efficiencies which compared favorably with classic calcium phosphate precipitation (CaP) procedures and lipofection.

    Article Title: Tricalcium phosphate nanoparticles enable rapid purification, increase transduction kinetics, and modify the tropism of mammalian viruses.
    Article Snippet: Adenoviral, adeno-associated viral, and retroviral particles are chosen as gene delivery shuttles in more than 50% of all gene therapy clinical trials.. Bulk availability of clinical-grade viral particles and their efficiency to transduce the therapeutic cargo into specific target cells remain the most critical bottlenecks in gene therapy applications to date.. Capitalizing on the flame-spray technology for the reproducible economic large-scale production of amorphous tricalcium phosphate nanoparticulate powders (ATCP), we designed a scalable ready-to-use gravity-flow column set-up for the straightforward concentration and purification of transgenic adenoviral, adeno-associated viral, and lentiviral particles.

    Virus:

    Article Title: In vitro evaluation of anti-HIV radioimmunoconjugates labeled with astatine-211, thorium-227 and actinium-225.
    Article Snippet: We conducted an in vitro investigation of the selective cytotoxicity of alpha-emitting radioimmunoconjugates (α-RICs) directed against cells expressing HIV envelope (Env) proteins.. It is well known that monoclonal antibody (mAb)-targeted α-emitting radionuclides can effectively kill antigen-expressing cells; however, the expected lowlevel expression of HIV antigens on latently infected cells poses an obstacle to all anti-HIV immune-based treatments, including α-RICs.. This investigation tested the cytotoxicity of the HIV envelope antigen-binding mAbs, PGT126 (binding gp120) and 7B2 (binding gp41), conjugated with labeling chelators that bind the α-emitters astatine-211 (211At), actinium-225 (225Ac) or thorium-227 (227Th).

    Article Title: A novel system for trigger-controlled drug release from polymer capsules.
    Article Snippet: Article history: Technologies currently avai Received 4 June 2008 Accepted 29 July 2008 Available online 5 August 2008

    Article Title: Heparin-binding EGF-like factor augments esophageal epithelial cell proliferation, migration and inhibits TRAIL-mediated apoptosis via EGFR/MAPK signaling.
    Article Snippet: Objective.. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been shown to stimulate the growth and migration of human keratinocytes in an autocrine or paracrine manner.. Bearing in mind the preceding narratives, present study was designed to explore the role of HB-EGF on esophageal epithelial cell growth, migration and anti-apoptosis.

    Article Title: Epstein-Barr Virus Thymidine Kinase Is a Centrosomal Resident Precisely Localized to the Periphery of Centrioles
    Article Snippet: .. 293 human embryonic kidney cells immortalized by sheared adenovirus (ATCC), 293T human embryonic kidney cells immortalized by sheared adenovirus and stably transfected with simian virus 40 (SV40) large T antigen (ATCC), and 143B TK , a human rhabdomyosarcoma cell line that lacks human TK-1 expression (ATCC), were all grown in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin. .. Chinese hamster ovary K1 (CHO-K1) cells (ATCC) were grown in F-12 medium and similarly supplemented.

    Article Title: Exploring nutrient supplementation and bioprocess optimization to improve the production of lentiviral vectors in serum-free medium suspension cultures.
    Article Snippet: .. Human embryonic kidney 293 cells, expressing the simian virus 40 (SV40) large T antigen, 293T cells (ATCC CRL-3216), were obtained from the American Type Culture Collection (Manassas, VA). ..

    Article Title: Inorganic nanoparticles for transfection of mammalian cells and removal of viruses from aqueous solutions.
    Article Snippet: Owing to their small size, synthetic nanoparticles show unprecedented biophysical and biochemical properties which may foster novel advances in life-science research.. Using flame-spray synthesis technology we have produced non-coated aluminum-, calcium-, cerium-, and zirconium-derived inorganic metal oxide nanoparticles which not only exhibit high affinity for nucleic acids, but can sequester such compounds from aqueous solution.. This non-covalent DNA-binding capacity was successfully used to transiently transfect a variety of mammalian cells including human, reaching transfection efficiencies which compared favorably with classic calcium phosphate precipitation (CaP) procedures and lipofection.

    Article Title: InXy and SeXy, compact heterologous reporter proteins for mammalian cells.
    Article Snippet: Mammalian reporter proteins are essential for gene-function analysis, drugscreening initiatives and as model product proteins for biopharmaceutical manufacturing.. Bacillus subtilis canmaintain its metabolism by secreting Xylanase A (XynA), which converts xylan into shorter xylose oligosaccharides.. XynA is a family 11 xylanase monospecific for D-xylose containing substrates.

    Article Title: Tricalcium phosphate nanoparticles enable rapid purification, increase transduction kinetics, and modify the tropism of mammalian viruses.
    Article Snippet: Adenoviral, adeno-associated viral, and retroviral particles are chosen as gene delivery shuttles in more than 50% of all gene therapy clinical trials.. Bulk availability of clinical-grade viral particles and their efficiency to transduce the therapeutic cargo into specific target cells remain the most critical bottlenecks in gene therapy applications to date.. Capitalizing on the flame-spray technology for the reproducible economic large-scale production of amorphous tricalcium phosphate nanoparticulate powders (ATCP), we designed a scalable ready-to-use gravity-flow column set-up for the straightforward concentration and purification of transgenic adenoviral, adeno-associated viral, and lentiviral particles.

    Transgenic Assay:

    Article Title: A novel system for trigger-controlled drug release from polymer capsules.
    Article Snippet: Article history: Technologies currently avai Received 4 June 2008 Accepted 29 July 2008 Available online 5 August 2008

    Article Title: Inorganic nanoparticles for transfection of mammalian cells and removal of viruses from aqueous solutions.
    Article Snippet: Owing to their small size, synthetic nanoparticles show unprecedented biophysical and biochemical properties which may foster novel advances in life-science research.. Using flame-spray synthesis technology we have produced non-coated aluminum-, calcium-, cerium-, and zirconium-derived inorganic metal oxide nanoparticles which not only exhibit high affinity for nucleic acids, but can sequester such compounds from aqueous solution.. This non-covalent DNA-binding capacity was successfully used to transiently transfect a variety of mammalian cells including human, reaching transfection efficiencies which compared favorably with classic calcium phosphate precipitation (CaP) procedures and lipofection.

    Article Title: InXy and SeXy, compact heterologous reporter proteins for mammalian cells.
    Article Snippet: Mammalian reporter proteins are essential for gene-function analysis, drugscreening initiatives and as model product proteins for biopharmaceutical manufacturing.. Bacillus subtilis canmaintain its metabolism by secreting Xylanase A (XynA), which converts xylan into shorter xylose oligosaccharides.. XynA is a family 11 xylanase monospecific for D-xylose containing substrates.

    Article Title: Tricalcium phosphate nanoparticles enable rapid purification, increase transduction kinetics, and modify the tropism of mammalian viruses.
    Article Snippet: Adenoviral, adeno-associated viral, and retroviral particles are chosen as gene delivery shuttles in more than 50% of all gene therapy clinical trials.. Bulk availability of clinical-grade viral particles and their efficiency to transduce the therapeutic cargo into specific target cells remain the most critical bottlenecks in gene therapy applications to date.. Capitalizing on the flame-spray technology for the reproducible economic large-scale production of amorphous tricalcium phosphate nanoparticulate powders (ATCP), we designed a scalable ready-to-use gravity-flow column set-up for the straightforward concentration and purification of transgenic adenoviral, adeno-associated viral, and lentiviral particles.

    Cell Culture:

    Article Title: Heparin-binding EGF-like factor augments esophageal epithelial cell proliferation, migration and inhibits TRAIL-mediated apoptosis via EGFR/MAPK signaling.
    Article Snippet: Objective.. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been shown to stimulate the growth and migration of human keratinocytes in an autocrine or paracrine manner.. Bearing in mind the preceding narratives, present study was designed to explore the role of HB-EGF on esophageal epithelial cell growth, migration and anti-apoptosis.

    Recombinant:

    Article Title: Heparin-binding EGF-like factor augments esophageal epithelial cell proliferation, migration and inhibits TRAIL-mediated apoptosis via EGFR/MAPK signaling.
    Article Snippet: Objective.. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been shown to stimulate the growth and migration of human keratinocytes in an autocrine or paracrine manner.. Bearing in mind the preceding narratives, present study was designed to explore the role of HB-EGF on esophageal epithelial cell growth, migration and anti-apoptosis.

    Stable Transfection:

    Article Title: Epstein-Barr Virus Thymidine Kinase Is a Centrosomal Resident Precisely Localized to the Periphery of Centrioles
    Article Snippet: .. 293 human embryonic kidney cells immortalized by sheared adenovirus (ATCC), 293T human embryonic kidney cells immortalized by sheared adenovirus and stably transfected with simian virus 40 (SV40) large T antigen (ATCC), and 143B TK , a human rhabdomyosarcoma cell line that lacks human TK-1 expression (ATCC), were all grown in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin. .. Chinese hamster ovary K1 (CHO-K1) cells (ATCC) were grown in F-12 medium and similarly supplemented.

    Transfection:

    Article Title: Epstein-Barr Virus Thymidine Kinase Is a Centrosomal Resident Precisely Localized to the Periphery of Centrioles
    Article Snippet: .. 293 human embryonic kidney cells immortalized by sheared adenovirus (ATCC), 293T human embryonic kidney cells immortalized by sheared adenovirus and stably transfected with simian virus 40 (SV40) large T antigen (ATCC), and 143B TK , a human rhabdomyosarcoma cell line that lacks human TK-1 expression (ATCC), were all grown in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin. .. Chinese hamster ovary K1 (CHO-K1) cells (ATCC) were grown in F-12 medium and similarly supplemented.

    Expressing:

    Article Title: Epstein-Barr Virus Thymidine Kinase Is a Centrosomal Resident Precisely Localized to the Periphery of Centrioles
    Article Snippet: .. 293 human embryonic kidney cells immortalized by sheared adenovirus (ATCC), 293T human embryonic kidney cells immortalized by sheared adenovirus and stably transfected with simian virus 40 (SV40) large T antigen (ATCC), and 143B TK , a human rhabdomyosarcoma cell line that lacks human TK-1 expression (ATCC), were all grown in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated calf serum, 100 U/ml penicillin, and 100 g/ml streptomycin. .. Chinese hamster ovary K1 (CHO-K1) cells (ATCC) were grown in F-12 medium and similarly supplemented.

    Article Title: Exploring nutrient supplementation and bioprocess optimization to improve the production of lentiviral vectors in serum-free medium suspension cultures.
    Article Snippet: .. Human embryonic kidney 293 cells, expressing the simian virus 40 (SV40) large T antigen, 293T cells (ATCC CRL-3216), were obtained from the American Type Culture Collection (Manassas, VA). ..



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